Response of the gypsy moth, Lymantria dispar to transgenic poplar, Populus simonii x P. nigra, expressing fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide.
Identifieur interne : 003138 ( Main/Exploration ); précédent : 003137; suivant : 003139Response of the gypsy moth, Lymantria dispar to transgenic poplar, Populus simonii x P. nigra, expressing fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide.
Auteurs : Chuan-Wang Cao [République populaire de Chine] ; Gui-Feng Liu ; Zhi-Ying Wang ; Shan-Chun Yan ; Ling Ma ; Chuan-Ping YangSource :
- Journal of insect science (Online) [ 1536-2442 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Aphides (MeSH), Endotoxines (génétique), Endotoxines (métabolisme), Femelle (MeSH), Hémolysines (génétique), Hémolysines (métabolisme), Larve (MeSH), Mâle (MeSH), Papillons de nuit (physiologie), Populus (génétique), Populus (métabolisme), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Régulation de l'expression des gènes végétaux (physiologie), Sexe-ratio (MeSH), Venins d'araignée (génétique), Venins d'araignée (métabolisme).
- MESH :
- génétique : Endotoxines, Hémolysines, Populus, Protéines bactériennes, Protéines recombinantes, Venins d'araignée.
- métabolisme : Endotoxines, Hémolysines, Populus, Protéines bactériennes, Protéines recombinantes, Venins d'araignée.
- physiologie : Papillons de nuit, Régulation de l'expression des gènes végétaux.
- Animaux, Aphides, Femelle, Larve, Mâle, Sexe-ratio.
English descriptors
- KwdEn :
- Animals (MeSH), Aphids (MeSH), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Endotoxins (genetics), Endotoxins (metabolism), Female (MeSH), Gene Expression Regulation, Plant (physiology), Hemolysin Proteins (genetics), Hemolysin Proteins (metabolism), Larva (MeSH), Male (MeSH), Moths (physiology), Populus (genetics), Populus (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sex Ratio (MeSH), Spider Venoms (genetics), Spider Venoms (metabolism).
- MESH :
- chemical , genetics : Bacterial Proteins, Endotoxins, Hemolysin Proteins, Recombinant Proteins, Spider Venoms.
- chemical , metabolism : Bacterial Proteins, Endotoxins, Hemolysin Proteins, Recombinant Proteins, Spider Venoms.
- genetics : Populus.
- metabolism : Populus.
- physiology : Gene Expression Regulation, Plant, Moths.
- Animals, Aphids, Female, Larva, Male, Sex Ratio.
Abstract
The response of the Asian gypsy moth Lymantria dispar (L.) (Lepidoptera: Lymantriidae) to a fusion gene consisting of the spider, Atrax robustus Simon (Araneae: Hexanthelidae) ω-ACTX-Ar1 sequence coding for an ω-atracotoxin and a sequence coding for the Bt-toxin C-peptide, expressed in transgenic poplar Populus simonii x P. nigra L. (Malphigiales: Salicaceae) was investigated. Individual performance, feeding selection, midgut proteinase activity and nutrition utilization were monitored. The growth and development of L. dispar were significantly affected by continually feeding on the transgenic poplar, with the larval instars displaying significantly shorter developmental times than those fed on nontransgenic poplar, but pupation was delayed. Mortality was higher in populations fed transgenic poplar leaves, than for larvae fed nontransgenic poplar leaves. The cumulative mortality during all stages of larvae fed transgenic leaves was 92% compared to 16.7% of larvae on nontransgenic leaves. The highest mortality observed was 71.7% in the last larval instar stage. A two-choice test showed that fifth-instar larvae preferred to feed on nontransgenic leaves at a ratio of 1:1.4. Feeding on transgenic leaves had highly significant negative effects on relative growth of larvae, and the efficiency of conversion of ingested and digested food. Activity of major midgut proteinases was measured using substrates TAME and BTEE showed significant increases in tryptase and chymotrypsinlike activity (9.2- and 9.0-fold, respectively) in fifth-instar larvae fed on transgenic leaves over control. These results suggest transgenic poplar is resistant to L. dispar, and the mature L. dispar may be weakened by the transgenic plants due to Bt protoxins activated by elevated major midgut proteinase activity. The new transgenic poplar expressing fusion protein genes of Bt and a new spider insecticidal peptide are good candidates for managing gypsy moth.
DOI: 10.1673/031.010.20001
PubMed: 21268699
PubMed Central: PMC3029309
Affiliations:
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(Lepidoptera: Lymantriidae) to a fusion gene consisting of the spider, Atrax robustus Simon (Araneae: Hexanthelidae) ω-ACTX-Ar1 sequence coding for an ω-atracotoxin and a sequence coding for the Bt-toxin C-peptide, expressed in transgenic poplar Populus simonii x P. nigra L. (Malphigiales: Salicaceae) was investigated. Individual performance, feeding selection, midgut proteinase activity and nutrition utilization were monitored. The growth and development of L. dispar were significantly affected by continually feeding on the transgenic poplar, with the larval instars displaying significantly shorter developmental times than those fed on nontransgenic poplar, but pupation was delayed. Mortality was higher in populations fed transgenic poplar leaves, than for larvae fed nontransgenic poplar leaves. The cumulative mortality during all stages of larvae fed transgenic leaves was 92% compared to 16.7% of larvae on nontransgenic leaves. The highest mortality observed was 71.7% in the last larval instar stage. A two-choice test showed that fifth-instar larvae preferred to feed on nontransgenic leaves at a ratio of 1:1.4. Feeding on transgenic leaves had highly significant negative effects on relative growth of larvae, and the efficiency of conversion of ingested and digested food. Activity of major midgut proteinases was measured using substrates TAME and BTEE showed significant increases in tryptase and chymotrypsinlike activity (9.2- and 9.0-fold, respectively) in fifth-instar larvae fed on transgenic leaves over control. These results suggest transgenic poplar is resistant to L. dispar, and the mature L. dispar may be weakened by the transgenic plants due to Bt protoxins activated by elevated major midgut proteinase activity. The new transgenic poplar expressing fusion protein genes of Bt and a new spider insecticidal peptide are good candidates for managing gypsy moth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21268699</PMID><DateCompleted><Year>2011</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1536-2442</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><PubDate><Year>2010</Year></PubDate></JournalIssue><Title>Journal of insect science (Online)</Title><ISOAbbreviation>J Insect Sci</ISOAbbreviation></Journal><ArticleTitle>Response of the gypsy moth, Lymantria dispar to transgenic poplar, Populus simonii x P. nigra, expressing fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide.</ArticleTitle><Pagination><MedlinePgn>200</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1673/031.010.20001</ELocationID><Abstract><AbstractText>The response of the Asian gypsy moth Lymantria dispar (L.) (Lepidoptera: Lymantriidae) to a fusion gene consisting of the spider, Atrax robustus Simon (Araneae: Hexanthelidae) ω-ACTX-Ar1 sequence coding for an ω-atracotoxin and a sequence coding for the Bt-toxin C-peptide, expressed in transgenic poplar Populus simonii x P. nigra L. (Malphigiales: Salicaceae) was investigated. Individual performance, feeding selection, midgut proteinase activity and nutrition utilization were monitored. The growth and development of L. dispar were significantly affected by continually feeding on the transgenic poplar, with the larval instars displaying significantly shorter developmental times than those fed on nontransgenic poplar, but pupation was delayed. Mortality was higher in populations fed transgenic poplar leaves, than for larvae fed nontransgenic poplar leaves. The cumulative mortality during all stages of larvae fed transgenic leaves was 92% compared to 16.7% of larvae on nontransgenic leaves. The highest mortality observed was 71.7% in the last larval instar stage. A two-choice test showed that fifth-instar larvae preferred to feed on nontransgenic leaves at a ratio of 1:1.4. Feeding on transgenic leaves had highly significant negative effects on relative growth of larvae, and the efficiency of conversion of ingested and digested food. Activity of major midgut proteinases was measured using substrates TAME and BTEE showed significant increases in tryptase and chymotrypsinlike activity (9.2- and 9.0-fold, respectively) in fifth-instar larvae fed on transgenic leaves over control. These results suggest transgenic poplar is resistant to L. dispar, and the mature L. dispar may be weakened by the transgenic plants due to Bt protoxins activated by elevated major midgut proteinase activity. The new transgenic poplar expressing fusion protein genes of Bt and a new spider insecticidal peptide are good candidates for managing gypsy moth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Chuan-Wang</ForeName><Initials>CW</Initials><AffiliationInfo><Affiliation>Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Gui-Feng</ForeName><Initials>GF</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhi-Ying</ForeName><Initials>ZY</Initials></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Shan-Chun</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Ling</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chuan-Ping</ForeName><Initials>CP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Insect Sci</MedlineTA><NlmUniqueID>101096396</NlmUniqueID><ISSNLinking>1536-2442</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004731">Endotoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009036" MajorTopicYN="N">Moths</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012744" MajorTopicYN="N">Sex Ratio</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013111" MajorTopicYN="N">Spider Venoms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>1</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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first="Zhi-Ying" last="Wang">Zhi-Ying Wang</name><name sortKey="Yan, Shan Chun" sort="Yan, Shan Chun" uniqKey="Yan S" first="Shan-Chun" last="Yan">Shan-Chun Yan</name><name sortKey="Yang, Chuan Ping" sort="Yang, Chuan Ping" uniqKey="Yang C" first="Chuan-Ping" last="Yang">Chuan-Ping Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Cao, Chuan Wang" sort="Cao, Chuan Wang" uniqKey="Cao C" first="Chuan-Wang" last="Cao">Chuan-Wang Cao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21268699
|texte= Response of the gypsy moth, Lymantria dispar to transgenic poplar, Populus simonii x P. nigra, expressing fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide.
}}
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